Catalog Number - 40031-SP50

40031-SP50 Cogent UDA™ (weak cation exchange, WCX) Semi‑Prep HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering a uniquely powerful separation mechanism that combines reversed phase, weak cation exchange, and HILIC retention in a single stationary phase.

The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon hydrophobic chain with a terminal carboxylic acid functional group, enabling:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This multi‑mode selectivity is especially valuable in semi‑preparative purification workflows, where difficult separations of polar, ionizable, and structurally similar compounds are required.

The TYPE‑C silica hydride base provides key advantages over conventional silica:

• Faster equilibration between runs

• Reduced sensitivity to mobile phase moisture (ideal for HILIC)

• Superior robustness and longer column life

• Lower salt requirements in HILIC mode (MS‑friendly purification)

50 mm Length – Fast Semi‑Prep Purification

The 50 mm length is optimized for speed and throughput in semi‑preparative applications.

• Very fast purification cycles

• Reduced solvent consumption

• Ideal for method scouting and rapid scale‑up

✅ Recommended for semi‑prep purification, high‑throughput workflows, and early-stage scale‑up

Flow Rate & System Requirements (10 mm ID, 4 µm)

⚠️ Requires semi‑preparative HPLC system capability

• Typical flow rate range: ~4 – 12 mL/min

• Moderate backpressure (4 µm particles)

• Designed for higher flow, semi‑prep operation

• Compatible with fraction collectors and prep detectors

✅ Important: A specialized HPLC system is required for columns of this size

✅ Best Practice: Use proper sample preparation and a matched guard column to protect the column and maintain performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Challenging Separations

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Controlled Retention

• WCX functionality active at pH ≥ 6

• Selectivity can be tuned via pH and ionic strength

✅ Ideal when traditional prep C18 or ion exchange columns cannot achieve target purity

TYPE‑C Silica Advantages

Faster Equilibration

• Improves cycle time in prep workflows

No Strict Moisture Control (HILIC)

• Simplifies mobile phase handling

Robust Column Lifetimes

• Durable under repeated preparative use

Low Salt Elution (HILIC Mode)

• Cleaner fractions and improved downstream processing

Cogent UDA™ Column Advantages

Mixed‑Mode Flexibility

• RP, HILIC, and WCX in one column

Semi‑Prep Scale Capability (10 mm ID)

• Suitable for purification and scale‑up

Balanced Performance (4 µm Particles)

• Efficient separations with manageable pressure

Typical Applications

• Semi‑preparative HPLC purification

• Scale‑up from analytical methods

• Complex matrix separations

• Basic and ionizable compound purification

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires semi‑preparative HPLC system capability

• Designed for higher flow operation (10 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

Please contact Customer Service for formal quotation, lead time, and ordering assistance.